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Postdoctoral Fellow in Carlos Fernandez-Hernando's Lab at Yale School of Medicine

(Former Ph.D. Student)

Wen Ding, Ph.D.

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Accomplishments of Wen in the Shehadeh Lab:

 

Publications:

1.       Ding W, Yousefi, K, and Shehadeh L (2018). Isolation, Characterization, and High Throughput Respiration Assay of Mouse Primary Renal Tubular Epithelial Cells. J Vis Exp. 2018 Jun 20;(136). doi: 10.3791/57718. PMID: 29985358.

2.       Ding W, Yousefi, K, Goncalves S, Goldstein B, Sabater A, Kloosterboer A, Ritter P, Lambert G, Mendez, A, and Shehadeh L (2018). Osteopontin Deficiency Ameliorates Alport Pathology by Preventing Tubular Metabolic Deficits. JCI Insight. 3(6):e94818. PMID: 29563333. Featured in Nature Reviews Nephrology. April 2018. PMID: 29643464.

3.       Kurtenbach S, Ding W, Goss GM, Hare JM, Goldstein BJ, and Shehadeh L. Differential Expression of MicroRNAs Among Cell Populations in the Regenerating Adult Mouse Olfactory Epithelium (2017). PLoS One.  Nov 6;12(11). PMID: 29107942. 

4.       Ding W, Shehadeh L (2017). New incriminating evidence against IGF2. Transl Cancer Res 2017;6(Suppl 6):S949-S952doi: 10.21037/tcr.2017.06.07. 

5.       Li J*, Yousefi K*, Ding W, Singh J, Shehadeh L (2017). Osteopontin RNA aptamer prevents and treats pressure overload-induced heart failure. May 1;113(6):633-643. doi: 10.1093/cvr/cvx016. Cardiovascular Research. PMID: 28453726

6.       Ding W, Li J, Singh J, Alif R, Vazquez-Padron RI, Gomes SA, Hare JM, Shehadeh L (2015). miR-30e targets IGF2-regulated osteogenesis in bone marrow-derived mesenchymal stem cells, aortic smooth muscle cells, and ApoE-/- mice. Cardiovasc Res. 2015 Apr 1;106(1):131-42. doi: 10.1093/cvr/cvv030. Featured article (Editor’s Choice). PMID: 25678587.

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Awards:

1. 2015-2017: AHA Predoctoral Fellowship: Role of miR-30e in Cholesterol Synthesis. PI: Wen Ding.   

2. 2015: AHA-James and Donna Dickenson-Sublett Awards for the Advancement of Cardiovascular Research.

3. 2016: American Society of Nephrology Kidney STAR award.

4. 2016  Medical Faculty Association Travel Award at University of Miami Miller School of Medicine.

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Conference presentations:

  1. Wen Ding, Jihe Li and Lina A Shehadeh. MiR-30e Reduces Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells by Repressing IGF2 Expression. Poster at AHA Scientific Sessions. Circulation, 2014. 128:A15230.

  2. Wen Ding, Jihe Li, Samirah Gomes, Joshua Hare, Lina Shehadeh. MiR-30e reduces osteogenic differentiation of bone marrow derived mesenchymal stem cells by directly targeting IGF2. Presented at the International Society for Heart Research (ISHR) May 2014. Published in JMCC, 2014. P. S24.

  3. MiR-30e Targets IGF2-Regulated Osteogenesis in Bone Marrow Derived Mesenchymal Stem Cells, Aortic Smooth Muscle Cells, and APOE-/- Mice. Ding W, Li J, Singh J, Alif R, Vazquez-Padron R, Gomes S, Hare J, Shehadeh L. Poster at AHA Scientific Sessions. Circulation, 2014. 130: A18084.

  4. Keyvan Yousefi, Wen Ding, Jayanti Singh, and Lina Shehadeh. The Aging Col4a3-/- Alport mouse develops heart failure with preserved ejection fraction? UM Center on Aging Scientific Poster Session, April 2016, Miami, Florida, USA.

  5. Keyvan Yousefi, Wen Ding, Jayanti Singh, and Lina Shehadeh. Is the Col4a3-/- Alport mouse a novel model for heart failure with preserved ejection fraction? Basic Cardiovascular Sciences (BCVS) 2016 Scientific Sessions, 18-21 July, Phoenix, Arizona, USA.

  6. Wen Ding, Keyvan Yousefi, Jayanti Singh, Stefania Goncalves, Bradley Goldstein, and Lina Shehadeh. Osteopontin Deficiency Reduces Alport Pathology. Oral presentation at the American Society of Nephrology: Kidney Week 2016, 15-20 November, Chicago, Illinois, USA.

  7. Li J, Yousefi K, Ding W, Singh J, Shehadeh L (2017). Osteopontin RNA aptamer prevents and treats pressure overload-induced heart failure. Oral presentation at the Keystone Symposium on RNA Approaches in Cardiovascular Disease 2017, 25-30 March, Keystone, Colorado, USA.

  8. Keyvan Yousefi, Wen Ding, Guerline Lambert, and Lina Shehadeh. Osteopontin Deficiency Ameliorates Heart Failure with Preserved Ejection Fraction (HFpEF) Pathology by Upregulating Mitochondrial 2-Oxoglutarate Dehydrogenase Like (OGDHL) Enzyme. Basic Cardiovascular Sciences (BCVS) 2017 Scientific Sessions, 10-14 July, Portland, Oregon, USA.

  9. Wen Ding, Keyvan Yousefi, Stefania Goncalves, Bradley Goldstein, Armando Mendez, Alfonso Sabater, and Lina Shehadeh. Osteopontin Deficiency Ameliorates Alport Pathology by Preventing DNM3-mediated Cholesterol Influx and Mitochondrial Energetic Deficit in Renal Tubules. American Society of Nephrology. 2017, 3-5 November, New Orleans, Louisiana, USA.

  10. Keyvan Yousefi, Wen Ding, Dong I. Lee, Virginia Hahn, David A. Kass, and Lina A. Shehadeh. Osteopontin Deficiency Ameliorates Heart Failure with Preserved Ejection Fraction (HFpEF) by Upregulating Mitochondrial 2-Oxoglutarate Dehydrogenase Like (OGDHL) in a Renal Disease Mouse Model. 2018, January 15-19. Keystone, Colorado, USA.

  11. Sunil kumar, Benny, Merline, Eliana Martinez, Keyvan Yousefi, Wen Ding, Lina A. Shehadeh, and Karen Young.  Klotho Prevents Long-term Cardiopulmonary Dysfunction in Rats with Experimental Bronchopulmonary Dysplasia. Pediatric Academic Societies (PAS) 2018 Meeting, May 5 - 8, Toronto, Canada.

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Conference Chairing:

1. 2014: Co-Chair of Stem Cell & Gene Therapy Session

    International Society for Heart Research (ISHR) Annual Conference, Miami, FL.

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K1 PIC.jpeg

Clinical Scientist at Longeverion, LLC

(Former Ph.D. Student)

Accomplishments of K1 in the Shehadeh Lab:

 

Publications:

  1. Dual actions of β2AR-agonism confer protection against heart failure and renal dysfunction via inotropic and lusitropic effects and normalized cholesterol homeostasis in a mouse model of Alport Syndrome. Chahdi A*, Yousefi K*, Capcha J, Irion C, Lambert G, Shehadeh S, Dunkley J, Lee Y, Khan A, Ramic M, Andrade N, Zeier Z, Dykxhoorn D, Katsoufis C, Freundlich M, Hare JM, Nabity M, Rivera C, Lymperopoulos A, Keith A Webster, and Shehadeh LA*. Addressing Reviwers' Concerns. 2021.

  2. Dunkley JC*, Irion CI*, Yousefi K, Shehadeh SA, Webster KA, Goldberger JJ, and Shehadeh LA*. Carvedilol and Exercise Combination Therapy Improves Systolic but not Diastolic Function and Reduces Plasma Osteopontin in Alport Mice. AJP - Heart and Circulatory Physiology. 2021. PMID: 33769915..

  3. Kamiar A*, Yousefi K*, Dunkley JC*, Webster KA, and  Shehadeh LA*. β2-Adrenergic Receptor Agonism as a therapeutic strategy for kidney disease. AJP - Regulatory, Integrative and Comparative Physiology. Feb 2021. PMID: 33565369.

  4. Patel M*, Rodrigues D*, Yousefi K*, John-Williams K, Mendez AJ, Goldberg  R, Lymperopoulos A, Tamariz  LJ, Goldberger JJ, Myerburg RJ, Junttila J* and Shehadeh LA*. Osteopontin and LDLR are Upregulated in Hearts of Sudden Cardiac Death Victims with Heart Failure with Preserved Ejection Fraction and Diabetes Mellitus. Front Cardiovasc Med. 2020 Nov 30;7:610282. doi: 10.3389/fcvm.2020.610282. eCollection 2020. PMID: 33330671. PMCID: PMC7734052

  5. Ali MF, Venkatarayappa SKB, Benny M, Rojas C, Yousefi K, Shehadeh LA, Kulandavelu S, Sharma M, Da Silva N, Freundlich M, Abitbol CL, DeFreitas MJ, Young KC. Effects of Klotho supplementation on hyperoxia induced renal injury in a rodent model of postnatal nephrogenesis. Pediatric Research 2020. Feb 14. doi: 10.1038/s41390-020-0803-z. PMID: 32059229

  6. Benny M, Hernandez DR, Sharma M, Yousefi K, Kulandavelu S, Batlahally S, Zambrano R, Chen P, Martinez EC, Schmidt AF, Shehadeh LA, Vasquez-Padron RI, Wu S, Velazquez OC, Young KC. Neonatal hyperoxia exposure induces aortic biomechanical alterations and cardiac dysfunction in juvenile rats. Physiol Rep. 2020 Jan;8(1):e14334. doi: 10.14814/phy2.14334. PMID: 31925922

  7. Yousefi K, Julian Dunkley, and Shehadeh LA. A preclinical model for phenogroup 3 HFpEF. Aging (Albany NY). 2019 Jul 15. doi: 10.18632/aging.102102. PMID: 31305259.

  8. Yousefi K*, Irion CI*, Takeuchi LM, Ding W, Lambert G, Eisenberg T, Sukkar S, Granzier HL, Methawasin M, Lee DI, Hahn V, Kass DA,  Hatzistergos K, Hare JM, and Shehadeh LA. Osteopontin Promotes Left Ventricular Diastolic Dysfunction through a Mitochondrial Pathway. Journal of American College of Cardiology. 2019;73:2705–18. PMID: 31146816.

  9. Ritter P*, Yousefi K*, Ramirez J, Dykxhoorn D, Menez A, and Shehadeh L (2018). LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring. J. Vis. Exp. (141), e58564, doi:10.3791/58564 (2018). PMID: 30507918​.

  10. Ding W, Yousefi, K, and Shehadeh L (2018). Isolation, Characterization, and High Throughput Respiration Assay of Mouse Primary Renal Tubular Epithelial Cells. J Vis Exp. 2018 Jun 20;(136). doi: 10.3791/57718. PMID: 29985358.

  11. Ding W, Yousefi, K, Goncalves S, Goldstein B, Sabater A, Kloosterboer A, Ritter P, Lambert G, Mendez, A, and Shehadeh L (2018). Osteopontin Deficiency Ameliorates Alport Pathology by Preventing Tubular Metabolic Deficits. JCI Insight. 3(6):e94818. PMID: 29563333. Featured in Nature Reviews Nephrology. April 2018. PMID: 29643464.

  12. Li J*, Yousefi K*, Ding W, Singh J, Shehadeh L (2017). Osteopontin RNA aptamer prevents and treats pressure overload-induced heart failure. May 1;113(6):633-643. doi: 10.1093/cvr/cvx016. Cardiovascular Research. PMID: 28453726.

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Awards:

1. 2018-2020: AHA Predoctoral Fellowship: Role of OPN-OGDHL in HFpEF. PI: Keyvan Yousefi.

2. 2018: Best poster award in basic science at the Department of Medicine Research Day, University of Miami Miller                  School of Medicine.   

3. 2019: American Society of Nephrology Kidney STAR award.

4. 2019:  Medical Faculty Association Travel Award at University of Miami Miller School of Medicine.

5. 2020: Best Student Research Assistant Award at the University of Miami.

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Conference presentations:

  1. Keyvan Yousefi, Wen Ding, Jayanti Singh, and Lina Shehadeh. The Aging Col4a3-/- Alport mouse develops heart failure with preserved ejection fraction? UM Center on Aging Scientific Poster Session, April 2016, Miami, Florida, USA.

  2. Keyvan Yousefi, Wen Ding, Jayanti Singh, and Lina Shehadeh. Is the Col4a3-/- Alport mouse a novel model for heart failure with preserved ejection fraction? Basic Cardiovascular Sciences (BCVS) 2016 Scientific Sessions, 18-21 July, Phoenix, Arizona, USA.

  3. Wen Ding, Keyvan Yousefi, Jayanti Singh, Stefania Goncalves, Bradley Goldstein, and Lina Shehadeh. Osteopontin Deficiency Reduces Alport Pathology. Oral presentation at the American Society of Nephrology: Kidney Week 2016, 15-20 November, Chicago, Illinois, USA.

  4. Li J, Yousefi K, Ding W, Singh J, Shehadeh L (2017). Osteopontin RNA aptamer prevents and treats pressure overload-induced heart failure. Oral presentation at the Keystone Symposium on RNA Approaches in Cardiovascular Disease 2017, 25-30 March, Keystone, Colorado, USA.

  5. Keyvan Yousefi, Wen Ding, Guerline Lambert, and Lina Shehadeh. Osteopontin Deficiency Ameliorates Heart Failure with Preserved Ejection Fraction (HFpEF) Pathology by Upregulating Mitochondrial 2-Oxoglutarate Dehydrogenase Like (OGDHL) Enzyme. Basic Cardiovascular Sciences (BCVS) 2017 Scientific Sessions, 10-14 July, Portland, Oregon, USA.

  6. Wen Ding, Keyvan Yousefi, Stefania Goncalves, Bradley Goldstein, Armando Mendez, Alfonso Sabater, and Lina Shehadeh. Osteopontin Deficiency Ameliorates Alport Pathology by Preventing DNM3-mediated Cholesterol Influx and Mitochondrial Energetic Deficit in Renal Tubules. American Society of Nephrology. 2017, 3-5 November, New Orleans, Louisiana, USA.

  7. Keyvan Yousefi, Wen Ding, Dong I. Lee, Virginia Hahn, David A. Kass, and Lina A. Shehadeh. Osteopontin Deficiency Ameliorates Heart Failure with Preserved Ejection Fraction (HFpEF) by Upregulating Mitochondrial 2-Oxoglutarate Dehydrogenase Like (OGDHL) in a Renal Disease Mouse Model. 2018, January 15-19. Keystone, Colorado, USA.

  8. Sunil kumar, Benny, Merline, Eliana Martinez, Keyvan Yousefi, Wen Ding, Lina A. Shehadeh, and Karen Young.  Klotho Prevents Long-term Cardiopulmonary Dysfunction in Rats with Experimental Bronchopulmonary Dysplasia. Pediatric Academic Societies (PAS) 2018 Meeting, May 5 - 8, Toronto, Canada.

  9. Benny, Merline, Eliana Martinez, Keyvan Yousefi, Wen Ding, Lina A. Shehadeh, and Karen Young.  Neonatal oxygen exposure induces persistent mitochondrial dysfunction in adult rodent renal tubular epithelial cells. Pediatric Academic Societies (PAS) 2019 Meeting, April 27 - 30.Baltimore, Maryland.

  10. Benny, Merline, Eliana Martinez, Keyvan Yousefi, Lina A. Shehadeh, and Karen Young.  Neonatal oxygen exposure induces systemic vascular remodeling and alters aortic SDF-1/CXCR7 expression in adult rodents. Pediatric Academic Societies (PAS) 2019 Meeting, April 27 - 30. Baltimore, Maryland.

  11. Camila Irion, Krista John-Williams, Ahmed Chahdi, Keyvan Yousefi, Yanelys R. Fernandez, Konstantinos E. Hatzistergos, Joshua M. Hare, Keith Webster, and Lina A. Shehadeh. Osteopontin Regulates Adult Cardiomyocyte Division in a Mouse Model of Pressure Overload Induced Heart Failure. American Heart Association BCVS Conference. Boston, Ma. July 2019. Accepted for Oral Presentation and selected for poster presentation at AHA Scientific Sessions, Philadelphia, November 2019.

  12. Julian C. Dunkley, Camila I. Irion, Keyvan Yousefi, Serene A. Shehadeh, Jeffrey J. Goldberger, and Lina A. Shehadeh. Treatment of a HFpEF mouse model with carvedilol, salbutamol, or exercise. ACC Works Congress of Cardiology. Chicago, Illinois. March 2020. Poster Presentation.

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Keyvan Yousefi, PharmD, Ph.D.

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Julian Dunkley .jpg

Medical Resident at SUNY Downstate Health Sciences University
(Former Postoctoral Fellow)

Accomplishments of Julian in the Shehadeh Lab:

 

Publications:

  1. Dual actions of β2AR-agonism confer protection against heart failure and renal dysfunction via inotropic and lusitropic effects and normalized cholesterol homeostasis in a mouse model of Alport Syndrome. Chahdi A*, Yousefi K*, Capcha J, Irion C, Lambert G, Shehadeh S, Dunkley JC, Lee Y, Khan A, Ramic M, Andrade N, Zeier Z, Dykxhoorn D, Katsoufis C, Freundlich M, Hare JM, Nabity M, Rivera C, Lymperopoulos A, Keith A Webster, and Shehadeh LA*. Addressing Reviwers' Concerns. 2021.

  2. Dunkley JC*, Irion CI*, Yousefi K, Shehadeh SA, Webster KA, Goldberger JJ, and Shehadeh LA*. Carvedilol and Exercise Combination Therapy Improves Systolic but not Diastolic Function and Reduces Plasma Osteopontin in Alport Mice. AJP - Heart and Circulatory Physiology. 2021. PMID: 33769915..

  3. Kamiar A*, Yousefi K*, Dunkley JC*, Webster KA, and  Shehadeh LA*. β2-Adrenergic Receptor Agonism as a therapeutic strategy for kidney disease. AJP - Regulatory, Integrative and Comparative Physiology. Feb 2021. PMID: 33565369.

  4. Yousefi K, Julian Dunkley, and Shehadeh LA. A preclinical model for phenogroup 3 HFpEF. Aging (Albany NY). 2019 Jul 15. doi: 10.18632/aging.102102. PMID: 31305259.

Awards:

1. 2018-2020: NIH Diversity Reseach Supplement Award. Role of OPN-OGDHL in HFpEF.

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Conference presentations:

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  1. Julian C. Dunkley, Camila I. Irion, Keyvan Yousefi, Serene A. Shehadeh, Jeffrey J. Goldberger, and Lina A. Shehadeh. Treatment of a HFpEF mouse model with carvedilol, salbutamol, or exercise. ACC Works Congress of Cardiology. Chicago, Illinois. March 2020. Poster Presentation.

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